NMR Spectroscopy, 3 op

Core content

• Physical basis of NMR spectroscopy.
  
• Instrumentation and principles of data acquisition and processing.
  
• Experimental setup for 1H and 13C spectra by pulsed methods.
  
• Interpretation of the first-order 1H and 13C spectra based on chemical shifts and coupling constants. Determination of the structure of a simple unknown compound.
  
• Interpretation of APT/DEPT 13C spectra and 2D homo- and hetero-correlated spectra (H,H-COSY, H,C-HSQC, NOESY).
  
• Practical skills in setup and measurement of 1H and 13C NMR-spectra. and 2D spectra

• Determination of relaxation times.
  
• Decoupling principles, Nuclear Overhauser effect.
  
• Interpretation of higher order 1H and 13C spectra and determination of the structure of more complex unknown compounds.
  
• Complex 1D pulse sequences, COSY pulse sequence.
  

• Interpretation of higher order 1H and 13C spectra and determination of the structure of more complex unknown compounds.
  
• Polarization transfer, INEPT and HETCOR, indirect detection experiments HSQC HMBC